Assay formats and screening
Assay development capabilities
Polyphor has in-house expertise in assay development (primary and secondary assays) for a wide range of target classes, which are available for internal and collaborative projects. These target classes include:
- Protein-Protein Interactions (PPIs)
- G-coupled protein receptors
- Tyrosine receptor kinases
- Wnt pathway
- Proteases
- Ion channels
- Toll-like receptors
- Antimicrobials
The current screening assay formats include:
- Calcium Flux, β-arrestin and ligand binding for GPCR assays
- Chemotaxis and cell migration
- Fluorescent (FP, FRET), luminescence and colorimetric
- FLIPR screen
- Cell growth, proliferation and apoptosis assays
- Enzyme linked immunosorbent assays (ELISAs)
- Phage Display screening
- Biacore assays for protein, peptide and small molecule or fragment interaction
- Antibacterial compound profiling: in vitro susceptibility, killing time curves, biofilms, membrane lysis, in vitro resistance
Molecular biology capabilities include:
Cell transfection, transformation and transduction, cloning, protein expression and purification.
